Acetyl-CoA(AcCoA) is the metabolic intermediate which transfers carbon atoms to the TCA cycle for energy production and is the link between fat and carbohydrate metabolism.  When fatty acid levels are high, AcCoA levels rise beyond the energy requirements of the organism and the excess is converted to the ketone bodies acetone and β-hydroxybutyrate. AcCoA also contributes carbon such as histone acetylation and isoprenoid synthesis. BioVision has developed an easy, convenient assay to measure the AcCoA level in biological samples. In the assay, AcCoA is specifically utilized to generate products which react with OxiRed Probe to generate color (λ=570 nm) and fluorescence (Ex/Em=535/587 nm). The assay can detect 0.1 to 10 nmol of AcCoA in a variety of samples.