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Identification of clones that contain the desired insert is an essential step in molecular cloning procedure. Traditional methods for selecting the correct inserts require growing up individual colonies, isolating plasmid DNAs, and then digesting with restriction enzymes to analyze each individual clone. Such traditional procedures are very time consuming, laborious, and expensive. The new InsertFinderTM PCR Quick Screening Kit is designed to quickly screen large numbers of clone candidates using PCR technology. The procedure is so simple and straightforward. The proprietary cell lysis buffer provided with the kit can efficiently lyse cells without interfering with PCR reactions. PCR products can directly be analyzed on an agarose gel without enzyme digestions. The new procedure provides significant convenience for screening large numbers of colonies on the same day your colonies are ready.
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